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Production of new 3D scaffolds for bone tissue regeneration by rapid prototyping

  • Biomaterials Synthesis and Characterization
  • Original Research
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Abstract

The incidence of bone disorders, whether due to trauma or pathology, has been trending upward with the aging of the worldwide population. The currently available treatments for bone injuries are rather limited, involving mainly bone grafts and implants. A particularly promising approach for bone regeneration uses rapid prototyping (RP) technologies to produce 3D scaffolds with highly controlled structure and orientation, based on computer-aided design models or medical data. Herein, tricalcium phosphate (TCP)/alginate scaffolds were produced using RP and subsequently their physicochemical, mechanical and biological properties were characterized. The results showed that 60/40 of TCP and alginate formulation was able to match the compression and present a similar Young modulus to that of trabecular bone while presenting an adequate biocompatibility. Moreover, the biomineralization ability, roughness and macro and microporosity of scaffolds allowed cell anchoring and proliferation at their surface, as well as cell migration to its interior, processes that are fundamental for osteointegration and bone regeneration.

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Acknowledgments

Authors would like to acknowledge funding from FEDER (POCI-COMPETE 2020, project number 007491), Portuguese Science Foundation (FCT) (UID/Multi/00709) and QREN (Programa Mais Centro) (CENTRO-07-0224-FEDER-002014).

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    1. CICS-UBI—Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal

      R. Fradique, T. R. Correia, S. P. Miguel, K. D. de Sá, D. R. Figueira, A. G. Mendonça & I. J. Correia

    2. Department of Chemistry, University of Beira Interior, R. Marquês d’Ávila e Bolama, 6201-001, Covilhã, Portugal

      A. G. Mendonça

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    R. Fradique and T. R. Correia have contributed equally to this work.

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    10856_2016_5681_MOESM1_ESM.tif

    Optical images of human osteoblast cells seeded in the presence of the different materials after 4 and 7 days of incubation at a magnification of 100×. Supplementary material 1 (TIFF 5088 kb)

    XRD spectra of TCP powder and of the produced scaffolds. Supplementary material 2 (PNG 453 kb)

    XRD card of CDHA and respective spectra. Supplementary material 3 (TIFF 3137 kb)

    Supplementary material 4 (TIFF 4524 kb)

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    Fradique, R., Correia, T.R., Miguel, S.P. et al. Production of new 3D scaffolds for bone tissue regeneration by rapid prototyping. J Mater Sci: Mater Med 27, 69 (2016). https://doi.org/10.1007/s10856-016-5681-x

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